Under isocratic elution condition including pH of the buffer solution, separation voltage and the ratio of organic solvent in the buffer solution.

• 首先以等位冲堤的方式,寻找最佳的实验参数,其参数包含缓冲溶液酸碱值,操作电压及有机修饰剂在缓冲溶液中的比例。