Tubular basement membrane antigens were solubilized with 6 mol/L guanidine HCl. The soluble proteins were used as antigens in Western blot analysis to detect autoantibodies in sera from patients with TIN.

• 肾小管基底膜 (tubularbasementmembrane ,TBM )经超声粉碎细胞后得到 ,在经 6mol/L盐酸胍处理后即为TBM可溶性抗原。