Transmission electron microscope(TEM) was used to observe the ultrastructure changes of the HPFs. The changes of the cell cycle and apoptosis of HPFs were evaluated with flow cytometry(FCM).

• 方法:应用细胞培养技术、流式细胞术(FCM)分析5F对HPF作用不同时间其细胞周期及诱导凋亡的特征,电镜观测HPFs超微结构改变。