Transformant number was increased by 20-fold by REMI(2 500 transformants per microgrammol/Lol/Le DNA) and 70%-75% of them were stable. REMI technique would be very beneficial to the establishment of the genetic transformation system of Monascus sp..
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- 应用REMI技术,转化率提高约20倍,每微克DNA 2 500个转化子,70%25~75%25的转化子的稳定,非常适合于红曲霉的转化。