Transduced cells were cloned in G418 and expanded for analysis of IGF-1 transgene expression and its effect on the cell proliferation.

• 方法 以脂质体为载体，用携带有报告基因绿色荧光蛋白（Green fluorescent protein，GFP）的重组IGF-1转染体外培养的人RPE细胞系，经G418筛选，通过荧光显微镜观察转染情况；