Total RNA was isolated at various time points to detect the expression of MMP13 and Col-2 by the method of quantitative Real-time PCR.

• 方法取C3H10T1/2细胞加入rhBMP-2诱导成骨细胞的产生,Von Kossa染色观察细胞形态,同时在不同时间段抽取总RNA,利用Real-time PCR检测MMP13、Collagen2的表达情况。