To obtain the subcellular localization of NDR3, Ndr3 was subcloned into pEGFP_N1 vector, then transfected into COS_7 cells.

• 将其亚克隆入绿色荧光蛋白表达载体pEGFP_N1,用脂质体介导法转染COS_7细胞 ,确定其亚细胞定位。