To establish time-efficient and sensitive method for detection of the methylation of FMR1(fragile X mental retardation)and XIST(X chromosome inactivation)genes,genomic DNA was deaminated by sodium bisulfite.

• 建立一种快速、灵敏的检测脆性X智障基因(fragile X mental retardation；FMR1)、X染色体失活基因(Xchromosome inactivation；XIST)甲基化的方法.