To elucidate the molecular basis ofthe attenuation ofDLA-EIAV in virulence thus provide theory foundation fordesigning HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned andsequenced.

• 本研究以DLA-EIAV感染细胞总DNA及其亲本强毒EIAVL株感染马外周血液白细胞中DNA为模板，应用PCR方法分段扩增出DLA-EIAV和L株的前病毒，并将各段扩增产物克隆后进行测序。