Three pairs of primers were designed and synthesized according to the published literatures and a triple PCR was developed to amplify CPS2J, epf and mrp gene sequences of Streptococus suis Type 2, with the target sequeuces of 230bp, 570bp and 860bp.

• 根据发表文献,设计合成三对引物,建立三重PCR方法,分别扩增猪链球菌2型cps2J、epf,mrp基因,目标片断大小分别为:230bp、570bp、860bp。