Three different concentrations (0 25 mg/L, 0 5 mg/L, or 1 0 mg/L) of Girk2 and wv Girk2 expression plasmids were transfected into the CAD cells.

• 用不同浓度 (0 2 5mg/L ,0 5mg/L ,1 0mg/L)的Girk2cDNA(对照组 )和wvGirk2cDNA(实验组 )质粒转染CAD细胞后 ,观察两组基因对转染CAD细胞的数目、蛋白质合成、神经突生长等指标的影响 ;