This paper briefly reviewed some useful gene transfer techniques, including microinjection, liposome-mediated, electroporation, ES-mediated, virus-mediated, sperm-mediated and ovary-injection.

• 摘要简要介绍了几种比较常用的转基因方法，包括显微注射法、脂质体介导法、电转染法、胚胎干细胞法、病毒载体法、精子载体法、卵巢直接注射法。