This effect makes it possible to eliminate all tumor cells despite only a fraction of them expressing HSV1 -tk gene. In this study, A fusion gene encoding both HSV1-TK and hygromycin phosphotransferase was used as the therapeutic gene.

• 本研究用一种与潮霉素磷酸转移酶基因(hygromycinphosphotransferase gene，hph)相融合的HSV1-tk基因作为目的基因(称为HyTK基因)，构建了重组逆转录病毒载体LNCHyTK；