Third,when NT embryos were cultured in NCSU-23 medium(North Carolina State University-23 medium) ,the development rate(30.06%) was no significantly different from that in TCM199 containing cumulus single layer culture system(31.5%).

• 核移胚以胚胎培养液NCSU23(NorthCarolinaStateUniversity23medium；北卡洲立大学培养液23)及卵丘颗粒单层共培养体系进行分别培养；核移胚卵裂率无明显差异(30.；06%25；31