These results indicate that BFF treated with serum direct starvation or gradient starvation can efficiently arrest them at Go/Gi stage, and the optimum duration of serum starvation is 3d.

• 结果表明，血清直接饥饿和梯度饥饿法均能有效地使水牛胎儿成纤维细胞同步于G_0/G_1期； 血清直接饥饿处理细胞3d可取得较好的细胞周期同步化效果。