These resulted in a weak grapevine quality . Comparision of three ELISA methods for detection of GLRaV showed that DAS-ELISA,PTA-ELISA and PAS-ELISA could detect GLRaV and GFLV. DAS-ELISA could shorten the detection time.

• 检测是认识一种病害最基本的前提，通过对三种ELISA检测GLRaV和GFLV的比较认为，双抗夹心-ELISA(DAS-ELISA)、间接-ELISA(PTA-ELISA)和A蛋白夹心-ELISA(PAS-ELISA)都能检测GLRaV和GFLV。