These cDNA fragments were cloned into the plasmid pUCm-T. The result indicated that the seguence of the genome was obtained. The genome of AEV-NH937 composed of 7055 nucleotides, potentially encodes a polyprotein of 2134 amino acids.
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- 把它们分段克隆在pUCm-T载体上,经序列分析,获得了AEV-NH937毒株的基因组全序列及推导的氨基酸序列,基因组全长为7055个核苷酸,与calnek疫苗株具有94.;3%25的同源性,编码一个含2134个氨基酸的多聚蛋白。