There are several unique restriction sites on each selective marker,AhdI, PstI, PvuI sites on the marker of Apr and Psp, EcoRI, NcoI on the marker of Cmr.

• 该质粒具有Apr和Cmr两个抗性标记,在其氨苄青霉素抗性基因上有AhdI、PstI、PvuI插入位点,在氯霉素抗性基因上有Psp、EcoRI、NcoI插入位点。