Then, the optimization of fermentation technique was carried out both in Erlenmeyer flasks and 15 L agitating fermentor, and the amplifying of fermentation technique was implemented in 50 L agitating fermentor.

 
  • 利用15 L发酵罐进行了发酵动力学研究;确定了发酵的最佳终止时间; 利用50 L发酵罐中进行了中试放大和发酵条件正交优化试验;结果表明较合适的发酵条件为搅拌速度300 r min~-1、通气量1:1.;00 vvm、发酵温度27。
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