Then we transfected pcDNA3/prepro neurturin into COS 7 cells with lipofectamine, and identified the mRNA and protein expression of prepro NTN gene by Northern Blot and Western Blot respectively.

• 再将prepro neurturin基因克隆至 pcDNA3真核表达载体中 ,经Lipofectamine转染 ,在COS 7细胞中瞬间表达 ,经NorthernBlot和WesternBlot印迹分析 ,鉴定其mRNA和蛋白表达正确。