Then the plasmid was transfected into a mouse dendritic cell line, DC2.4, origining from C57BL/ 6 (B6) mouse, by LipofectAMINETM 2000. The expression of mBTIA mRNA and mBTLA-hIg fusion protein were determined by RT-PCR, ELBA and Western blot.

• 采用RT-PCR、ELISA和Western blot分别检测pmBTLA-hIg质粒转染DC中mBTLA基因mRNA的表达及细胞培养上清中mBTLA-hIg融合蛋白的表达;