Then the biological engineering strain WYBS2001T with human egf was obtained by transforming pUSE which was constructed by cloning egf into the secreting plasmid pUSl86, into mutant strain WYBS2001.

 
  • 然后将其克隆至枯草杆菌分泌型质粒载体pUSl86上构建成重组质粒pUSE,并转化枯草杆菌突变菌株WYBS2001,获得转入egf基因的枯草杆菌生态工程菌WYBS2001T。
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