The vitelline envelope was dissipated very quickly when eggs were spawned in Ca2+-free ASW and this breakdown was not triggered by the proteases.

• 从成熟卵巢中取出卵，于正常海水中培养，有的卵黄外膜不会崩解，胶体亦不会均质化，但是仍可释出极体，所以减数分裂的恢复进行与卵黄外膜的崩解及胶质层的形成机制无关。