The variable factors of liquid chromatography are composition and PH value of the mobile phase, same type of chromatographic column but from different manufacturers or different batches, column temperature, sampler and detector temperature, etc.

• 液相色谱法中典型的变动因素有：流动相的组成和pH、不同厂牌或不同批号的同类型色谱柱、固定相、柱温、进样器和检测器温度等。