The transformation principle Ko-p 77 which possessed the ability of producing kanamycin and forming spores was obtained when the plasmid-negative strain Ko 42-7 used as recipient was transformed with the kanamycin-producing strain Kp 958-4 as donor.

• 当把质粒消除株Ko42-7作为受体菌株,把产生卡那霉素产生菌Kp958-4作为供体菌株进行DNA转化时,获得了具有产生卡那霉素和形成孢子能力的转化子Ko-p77。