The time needed to reach equilibrium and the effect of temperature and concentration of methanol on distribution coefficient of free gossypol were also investigated.

• 结果表明,甲醇相中加入一定量的柠檬酸可以提高游离棉酚在甲醇相中的分配系数,而且温度越高,甲醇相浓度越大,分配系数越大。