The soluble fusion protein P25 was purified after expending cultivation by Ni-NTA affinity chromatography under the denaturing condition.

• 该P25融合蛋白以可溶形式存在，扩大培养按1:40的比例浓缩后在变性条件下经Ni离子亲和层析得到纯品P25融合蛋白。