The shoots differentiation rate of the callus cultured in differentiation medium (MSR 3c,MS +casamino acids 500 mg/L +glutamine 300 mg/L +proline 500 mg/L +mannitol 36.4 g/L+maltose 30 g/L+TDZ 0.2 mg/L +phytagel 5 g/L pH 5.8) was over 90%.

• 采用MSR3c (MS +casaminoacids 5 0 0mg/L +glutamine 30 0mg/L +proline 5 0 0mg/L +mannitol 36 4 g/L +maltose 30 g/L +TDZ 0 2mg/L +phytagel 5 g/LpH5 8)为分化培养基 ,愈伤组织分化率均在 90%25以上。