The sexual distinction method of young frog was presented in this paper.The fixation of young flog, draw of gonads, procession of making slices and sexual distinction were described in details.

• 针对刚变态的中国林蛙幼蛙雌雄不易鉴定的问题，本研究找出了幼蛙的固定、生殖腺的剖取、性腺组织切片的制作过程及性别鉴定方法。