The results show that methotrexate strongly quenched the fluorescence of bovine serum albumin,and the quenching mechanism is a static quenching procedure.

• 结果表明:在生理条件下,甲氨喋呤对牛血清白蛋白荧光有较强的猝灭作用,其猝灭方式为静态猝灭。