The results of restriction and PCR showed the insertion was right and the recombinant adenovirus generated contained the CD and TK fusion gene without replication competent adenovirus.

• 酶切结果及 PCR结果显示 ,重组粘粒中 CD、TK融合基因插入正确 ,经鉴定所获重组腺病毒带有融合基因 ,并且无具有复制能力的腺病毒存在。