The reendothelization level on the surface of the leaflets was 92% before pulsatile-flow-cultivation. But the rate of ECs retention on the scaffold exposured to pulsatile flow was only 36%, 23% and 11% on the 7th, 10th and 14th day, respectively.

• 在脉动流培养前,组织工程瓣内皮化程度为92%25,而脉动流培养的第7、10、14d,其ECs黏附率分别为36%25、23%25和11%25,说明种植的ECs脱落严重。