The reduction was conducted in Tris-HCl buffer, and the concentrations of COBE and CHBE and the optical purity of product were determined by capillary GC and HPLC equipped with a chiralcel OB column, respectively. The product was mainly D-(S)-CHBE.

 
  • 实验发现 ;在水相体系中主要生成 D (S) 型产物 ;COBE的转化率及CHBE的收率和光学选择性都比较高 .
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