The recombinant Escherichia coli JM109 strain harboring the expression plasmid was induced by IPTG, gained the rCR protein band by SDS-PAGE analysis, and the specific activity of the E.

• coli JM109/pTrc-rCR; IPTG诱导重组蛋白的表达,SDS-PAGE分析得到重组蛋白表达条带;