The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28% (and 35%).

• VH和VL重组质粒分别转化到大肠肝菌BL21（DE3）中，IPTG诱导后，经SDS?PAGE电泳表明在12kD处有包含体蛋白表达，表达蛋白含量分别为28%25和35%25。