The rabbits were immunized with Ab1 and part of the rabbit serum containing ani-Id (Ab2)were salted out with (NH_4)_2SO_4, then anti-Id were purified with Sepharose-4B affinityfiltration.

• 将此IgG作为免疫原免疫家兔，制得的兔抗血清中含抗-Id（Ab_2）。 部分兔抗血清经（NH_4）_2SO_4盐析后，再经亲和层析吸收纯化，得纯化抗-Id。