The purpose of this study was to develop an indirect ELISA to detect the paratuberculosis serum antibody in deer serum.

• 采用副结核亲和层析抗原,待检鹿血清以草分枝杆菌抗原吸收,建立用于检测鹿副结核病血清抗体的间接ELISA诊断方法。