The purpose of the experiment is to construct pcDNA3 expression vectors containing p27 gene by PCR and endonuclease lysis.

• 本实验的目的在于通过PCR、酶切等步骤构建含有p27基因的载体pcDNA3，然后通过脂质体转染进入肿瘤细胞MCF7中，筛选到稳定表达株。