The purified sporulated oocysts were sonicated at a power setting of 200 W,durative time 5s,interval time Ss,60 times,and 3 cycle.

• 纯化的孢子化的卵囊用超声波裂解，功率设置为200W、工作时间5秒、间隙时间5秒、工作次数60次，3个循环。