The protein level of p27 Klpl increased at Ih , decreased from 6 to 24h after 2Gy, it decreased from 3 to 24 h after lOGy, and the level of p27Kipl associated with Cdk2 decreased, then lost its inhibition function to cyclin-dependent kinase.

• cyclinE蛋白表达水平在ZGy照射后略有增加，1 OGy照射后lh开始增加，持续到24h，但IR后cdkZ蛋白水平未发生变化，通过免疫共沉淀检测了10Gy照射后edkZ与cydinE结合，发现随着照射时间的延长，cyclinE与cdkZ结合量增多，这说明cyclinE蛋白水平的增加能反映cyclin E/CDKZ的激酶活性。