The proportions of peripheral CD4~+CD28~-T cells and CD4~+CD25~+ Treg subsets were determined with fluorescence-activated cell sorting (FACS).

• 借助三色荧光标记技术对外周血CD4~+CD28~-T细胞和CD4~+CD25~+Treg占CD4~+T细胞比例进行流式细胞术测定。