The optional subculture medium is MS basal medium with 2mg/L 2,4-D,40g/L sucrose and lOg/L agar. High sucrose and agar is apt to keep the tight close structure of callus.

• 最佳继代培养基为：MS+2mg/L 2,4-D+40g/L蔗糖+10g/L琼脂，提高蔗糖及琼脂浓度有利于愈伤组织保持结构紧密的状态。