The optimal results of this staining procedure were obtained under the conditions of 30 rain BrdUrd labeling time, 2.4 mol/L HC1, 30 min HC1 treatment, 1 hour incubation of the monoclonal antibody to BrdUrd and without RNase treatment.

• 在30分钟BrdUrd标记时间；2.；4mol/L盐酸30分钟处理时间及抗BrdUrd单克隆抗体20℃1小时温育时间等条件下；可获得满意的染色结果；并且不需要核糖核酸酶的处理。