The optimal RSA addition method was performed. P -cyclodextrin (P -cyclodextrins/RSA 1:1, mole ratio) was added to RSA blended with 1.2% TweenSO, followed by a 20 minutes sterilization.

• 最终确定了适摘要宜的底物投料方式:向底物RSA与1 .；2%25吐温80的混悬液中加入p一环糊精(与RSA摩尔比为l:1)，湿热灭菌20min投料。