The mutant M14 of Corynebacteriumammoniagenes was obtained whose UMP yield was 7-fold higher than the parent strain R248.The conditions of fermentable and enzymatic conversion of M14 were optimized.
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- 采用链霉素抗性、卡那霉素抗性和产物结构类似物抗性等作为筛选手段,对产氨短杆菌R248 进行离子束和紫外诱变,选育出一株UMP 生成能力比出发菌株R248 提高7 倍突变株M14。 对高活性突变株M14 的细胞转化和发酵转化条件进行优化。