The molecular weight of the native lipase was estimated to be about 29 kD by gel filtration using Sephadex G 150, and that of the denatured lipase was determined to be about 27.5 kD by its mobility on SDS PAGE, indicating that the lipase was a monomer.

 
  • SDS?PAGE 和凝胶过滤分别测得酶的分子量为27.;5 kD和29 kD, 表明该酶以单体形式存在。
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