The molecular mass of the PLC was determined as 64 kDa using SDS-PAGE, which was in agreement with the results obtained from Sephadex G-200 column chromatography (65 kDa), suggesting that the PLC was a monomeric protein.

• SDS-PAGE电泳显示该磷脂酶C呈现单一条带,分子量约为64 kDa。 通过Sephadex G-200凝胶过滤层析柱标定的磷脂酶C的分子量为65 kDa,两者的结果一致,表明该酶是单体蛋白质。