The main result is that: it had a little of generative obstacle between R. amurensis and R. ciliaris or R. amurensis and R. japoensis, but no between R. ciliaris and R. japonensis.

• 结果表明毛叶鹅观草与纤毛鹅观草、竖立鹅观草存在一定的生殖障碍，纤毛鹅观草和竖立鹅观草不存在生殖障碍。