The luciferase activity of lysate expressing FLAG-pcDNA3-N was lower than that expressing FLAG-pcDNA3.Addition of N protein in RRL system decreased luciferase activity.

• 构建了载体FLAG-pcDNA3-N,在293T人胚肾细胞内表达后,荧光素酶活性被抑制; 在体外翻译体系中加入N蛋白,体外翻译被抑制。