The level of mRNA and protein in study group were estimated by reverse transcription polymerase chain reaction(RT-PCR), flow cytometric analysis(FCS) and immunohistochemistry individually.

• 分别用半定量逆转录-聚合酶连反应(reverse transcription-polymerase chain reaction，RT-PCR)、流式细胞仪(FCS)和免疫组化法检测ARL-1基因在转基因细胞(实验组)中的mRNA表达水平和蛋白表达水平。